Work with FFPE and fresh frozen tissue
Almac's proprietary discovery arrays (Xcel™ and DSA®) have each been designed to enable profiling from both frozen and FFPE tissue, to allow application of this unique microarray technology to current clinical practice and retrospective tissue banks.
 All of our proprietary discovery arrays (Xcel™ and Cancer DSA®) are designed with a 3' focus providing an optimised platform for the analysis of FFPE samples.
RNA extracted from FFPE samples tends to have a shorter median transcript length and the detection of these samples on generic arrays is highly unstable and non-reproducible. By designing probe sets specific to the 3' extremities of transcripts, a much higher detection rate is possible.
Some of our published papers and publications on FFPE:
Development of Expression Based Biomarkers in NSCLC: A Study of Intratumor Heterogeneity Using FFPE Tissue
Breast Cancer DSA: A platform for discovery of biomarkers from breast cancer derived FFPE tissue
RNA expression analysis from formalin fixed paraffin embedded tissues. Farragher SM, Tanney A, Kennedy RD, Paul Harkin D. Histochem Cell Biol. 2008 vol. 130, issue 3, pp 435-445
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